High-content & High-throughput Screening


Baustelle...

 

Literatur:

Talwar S, Lynch JW, Gilbert DF. Fluorescence-based high-throughput functional profiling of ligand-gated ion channels at the level of single cells. PLoS One. 2013;8(3):e58479. doi: 10.1371/journal.pone.0058479. Epub 2013 Mar 8. PubMed PMID: 23520514; PubMed Central PMCID: PMC3592791.

Jung J, Weisenburger S, Albert S, Gilbert DF, Friedrich O, Eulenburg V, Kornhuber J, Groemer TW. Performance of scientific cameras with different sensor  types in measuring dynamic processes in fluorescence microscopy. Microsc Res Tech. 2013 Aug;76(8):835-43. doi: 10.1002/jemt.22236. Epub 2013 Jun 3. Erratum in: Microsc Res Tech. 2013 Oct;76(10):1099. PubMed PMID: 23733589.

Gilbert DF, Erdmann G, Zhang X, Fritzsche A, Demir K, Jaedicke A, Muehlenberg K, Wanker EE, Boutros M. A novel multiplex cell viability assay for high-throughput RNAi screening. PLoS One. 2011;6(12):e28338. doi: 10.1371/journal.pone.0028338. Epub 2011 Dec 5. PubMed PMID: 22162763; PubMed Central PMCID: PMC3230607.

Gilbert DF, Islam R, Lynagh T, Lynch JW, Webb TI. High Throughput Techniques for Discovering New Glycine Receptor Modulators and their Binding Sites. Front Mol Neurosci. 2009 Oct 30;2:17. doi: 10.3389/neuro.02.017.2009. eCollection 2009. PubMed PMID: 19949449; PubMed Central PMCID: PMC2782790.

Gilbert DF, Wilson JC, Nink V, Lynch JW, Osborne GW. Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry. Cytometry A. 2009 May;75(5):440-9. doi: 10.1002/cyto.a.20703. PubMed PMID: 19184990.

Bartz F, Kern L, Erz D, Zhu M, Gilbert D, Meinhof T, Wirkner U, Erfle H, Muckenthaler M, Pepperkok R, Runz H. Identification of cholesterol-regulating genes by targeted RNAi screening. Cell Metab. 2009 Jul;10(1):63-75. doi: 10.1016/j.cmet.2009.05.009. PubMed PMID: 19583955.

Gilbert D, Esmaeili A, Lynch JW. Optimizing the expression of recombinant alphabetagamma GABAA receptors in HEK293 cells for high-throughput screening. J Biomol Screen. 2009 Jan;14(1):86-91. doi: 10.1177/1087057108328017. PubMed PMID: 19171924.

Gilbert DF, Meinhof T, Pepperkok R, Runz H. DetecTiff: a novel image analysis routine for high-content screening microscopy. J Biomol Screen. 2009 Sep;14(8):944-55. doi: 10.1177/1087057109339523. Epub 2009 Jul 29. PubMed PMID: 19641223.

Kruger W, Gilbert D, Hawthorne R, Hryciw DH, Frings S, Poronnik P, Lynch JW. A yellow fluorescent protein-based assay for high-throughput screening of glycine and GABAA receptor chloride channels. Neurosci Lett. 2005 Jun 3;380(3):340-5. Epub 2005 Feb 23. PubMed PMID: 15862914.